Corn (Zea mays L.) is an important crop that contributes to global food security, but understanding how farm management practices and soil health affect corn grain nutrient analysis and therefore human health is lacking. Leveraging Rodale Institute's Farming Systems Trial—a long-term field experiment established in 1981 in Kutztown, PA, USA—this study was conducted to assess the impact of different agricultural management systems on corn grain nutrient profiles in a long-term trial that has resulted in differences in soil health indicators between treatments as a result of long-term management. The main plot factor was two tillage practices (intensive and reduced) and the subplot factor was four cropping systems (non-diversified conventional [nCNV], diversified conventional [dCNV], legume-based organic [ORG-LEG], and manure-based organic [ORG-MNR]). Generally, the levels of amino acids, vitamins, and protein in corn grain were greatest in the ORG-MNR system, followed by the ORG-LEG and dCNV systems, and finally the nCNV system. It is important to consider that the observed difference between the organic and conventionally grown grain could be due to variations in corn hybrids that were used in those systems. However, nutrient composition of corn differed within cropping systems but between management practices (diversified crop rotation and cover cropping) which also contributed to differences in soil health indicators (soil compaction, soil protein, and organic C levels) that may also influence grain nutrient concentrations. With the exception of methionine, nutrient concentration in corn grain was not affected by different tillage regimes. These findings provide novel information on corn grain nutritional quality of organic and conventional cropping systems after long-term management and give insights into how system-specific components affect nutrient composition of corn grain.

Uncontrolled weeds can cause billions of dollars in yield loss in corn and soybean production fields in the United States. Growers often use herbicides to control weeds to avoid yield loss. The objective of this study was to estimate the cost of herbicides used in corn and soybean production in the United States. On-farm herbicide usage data were extracted from corn (2021) and soybean (2023) surveys of the United States Department of Agriculture–National Agricultural Statistics Service (USDA-NASS 2022a, 2024a) Agricultural Chemical Use Program. Commonly used or known products were assumed for each reported herbicide. Based on the USDA-NASS surveys, approximately 107.8 and 89.1 million kg of herbicides were applied in corn and soybean production in 2021 and 2023, respectively, in the United States. Glyphosate (33.8 million kg; in various salt forms), atrazine (26.8 million kg), and mesotrione (2.4 million kg) were the most widely applied herbicides, on 79%, 65%, and 47% of corn, respectively, planted in 2021 (USDA-NASS 2022b). Similarly, glyphosate (in various salt forms) was the most widely applied herbicide in soybean, followed by 2,4-D (15.9 million kg; in various salt forms) and glufosinate-ammonium (4.4 million kg), which were applied to 58% and 23% of soybean, respectively, planted in 2023 (USDA-NASS 2024c). Using the average retail price of herbicide products from Kansas State University, University of Nebraska−Lincoln, and North Dakota State University publications, herbicides (excluding adjuvants and application costs) would have cost approximately $3.2 and $4.1 billion in corn and soybean in 2021 and 2023, respectively.

Palmer amaranth with resistance to dicamba, glufosinate, and protoporphyrinogen oxidase inhibitors has been documented in several southern states. With extensive use of these and other herbicides in South Carolina, a survey was initiated in fall 2020 and repeated in fall 2021 and 2022 to determine the relative response of Palmer amaranth accessions to selected preemergence and postemergence herbicides. A greenhouse screening experiment was conducted in which accessions were treated with three preemergence (atrazine, S-metolachlor, and isoxaflutole) and six postemergence (glyphosate, thifensulfuron-methyl, fomesafen, glufosinate, dicamba, and 2,4-D) herbicides at the 1× and 2× use rates. Herbicides were applied shortly after planting (preemergence) or at the 2- to 4-leaf growth stage (postemergence). Percent survival was evaluated 5 to 14 d after application depending on herbicide activity. Sensitivity to atrazine preemergence was lower for 49 and 33 accessions out of 115 to atrazine applied preemergence at the 1× and 2× rate, respectively. Most of the accessions (90%) were controlled by isoxaflutole applied preemergence at the 1× rate. Response to S-metolachlor applied preemergence indicated that 34% of the Palmer amaranth accessions survived the 1× rate (>60% survival). Eleven accessions exhibited reduced sensitivity to fomesafen applied postemergence; however, these percentages were not different from the 0% survivor group. Glyphosate applied postemergence at the 1× rate did not control most accessions (79%). Palmer amaranth response to thifensulfuron-methyl applied postemergence varied across the accessions, with only 36% and 28% controlled at the 1× rate and 2× rate, respectively. All accessions were controlled by 2,4-D, dicamba, or glufosinate when they were applied postemergence. Palmer amaranth accessions from this survey exhibited reduced susceptibility to several herbicides commonly used in agronomic crops in South Carolina. Therefore, growers should use multiple management tactics to minimize the evolution of herbicide resistance in Palmer amaranth in South Carolina.

Following the application of MCPA/MCPB at 1.7 kg ae ha−1 at a field site near Dresden, ON, Canada, poor control (<50% visible control) of green pigweed (Amaranthus powellii S. Watson) was observed. Amaranthus powellii is a common weed in Ontario crop production, and its evolution of resistance to synthetic auxin herbicides (SAHs) could pose a risk to crop yields. The suspected resistant A. powellii population (R) was used in dose–response and field experiments to determine resistance to SAHs. The objective of these studies was to determine whether this population of A. powellii is resistant to MCPA and cross-resistant to other SAHs. The GR50 (herbicide dose that causes a 50% reduction in plant aboveground biomass) values were determined by fitting plant dry weight data, obtained following application with seven SAHs, to a four-parameter log-logistic equation and were compared between the suspected-resistant (R) population and a known susceptible (S) population of A. powellii. The field trial was conducted in 2017, 2018, 2019, and 2021 in corn (Zea mays L.) and consisted of 11 postemergence SAH treatments. The GR50 values differed between the R and S populations following application with MCPA, aminocyclopyrachlor, dichlorprop-p, and mecoprop, resulting in resistance factors of 4.4, 3.0, 2.5, and 2.4, respectively. In the field study, dicamba and MCPA ester controlled A. powellii 84% and 30%, respectively, at 8 wk after treatment application (WAA). The control of Amaranthus powellii with all SAHs applied POST in corn was poor (<90% visible control) at 8 WAA. Both studies confirmed resistance to SAHs in this population of A. powellii, which will create limitations for farmers aiming to control this weed.

Amicarbazone, atrazine, and metribuzin behavior was examined in a field setting in Tennessee and in a laboratory setting using soils collected from Illinois and Tennessee. Fields planted to corn were sampled from 0 to 8 cm depth, and the samples were analyzed using methanolic extraction followed by tandem mass spectrometry analysis to determine residual herbicide concentrations. Conditions were favorable for herbicide degradation, including warm temperatures and adequate rainfall. All herbicide half-lives were <10 d. Laboratory research using soils with known atrazine-use histories showed that amicarbazone did not exhibit enhanced microbial degradation due to previous atrazine use. Apparent amicarbazone and metribuzin persistence levels implied that early-season weed control would be expected, but carryover to injure sensitive rotational crops would not be anticipated under these environmental conditions. Dissipation under field conditions of amicarbazone and metribuzin was not affected by being applied to separate plots or by coapplication to the same plots.

Nebraska is one of the top five corn-growing states in the United States, with the planting of corn on 3.5 to 4 million hectares annually. Harvest loss of corn results in volunteer corn interference in the crop grown in rotation. Estimating the extent of harvest loss and expected volunteer corn density is a key to planning an integrated volunteer corn management program. This study aimed to evaluate the harvest loss of corn and estimate the potential for volunteerism. Harvest loss samples were collected after corn harvest from a total of 47 fields in six counties, including 26 corn fields in 2020, and 21 fields in 2021, in south-central and southeastern Nebraska. An individual cornfield size was 16 to 64 ha. A total of 16 samples were collected from each field after corn harvest in 2020 and 2021. Harvest loss of corn was 1.5% and 0.7% of the average yield of 15,300 kg ha−1 in 2020 and 2021, respectively. Corn harvest loss was 191 and 80 kg ha−1 from dryland fields, and 206 and 114 kg ha−1 from irrigated fields in 2020 and 2021, respectively. An average kernel loss of 68 and 33 m−2 occurred in 2020 and 2021, respectively. The germination percentage of corn kernels collected from harvest loss was 51%, which implies that volunteer corn plants of 35 and 17 m−2 from 2020 and 2021, respectively, could be expected in successive years. A volunteer corn management plan is required, because if it is not controlled, this level of volunteer corn density can cause yield reduction depending on the crop grown in rotation.

Prevented planting payments reimburse crop producers for losses from not being able to plant. These payments provide critical protection to producers; however, these payments, which are determined using a nationwide, crop-specific coverage factor, have been questioned to induce moral hazard. Depending on the region and crop insurance coverage, payments from this provision exceed producers’ losses. This paper estimates the prevented planting coverage factor by coverage level and region that would equitably reimburse corn and soybean producers for their losses. We find the prevented planting coverage factor has significant variation across coverage levels and location within our study region. The prevented planting coverage factor was found to decline as the policy coverage level increases. The further north in the study region the higher the coverage factor, likely due to increased land rent expenses. The results provide a unique perspective of how these coverage factors would vary to equitably compensate producers for losses, which addresses the moral hazard concerns with prevented planting.

Tiafenacil is a new nonselective protoporphyrinogen IX oxidase–inhibiting herbicide with both grass and broadleaf activity labeled for preplant application to corn, cotton, soybean, and wheat. Early season rice emergence and growth often coincide in the mid-southern United States with applications of preplant herbicides to cotton and soybean, thereby increasing the opportunity for off-target herbicide movement from adjacent fields. Field studies were conducted to identify any deleterious effects of reduced rates of tiafenacil (12.5% to 0.4% of the lowest labeled application rate of 24.64 g ai ha−1) applied to 1- or 3-leaf rice. Visual injury 1 wk after treatment (WAT) for the 1- and 3-leaf growth stages ranged from 50% to 7% and 20% to 2%, respectively, whereas at 2 WAT these respective ranges were 13% to 2%, and no injury was observed. Tiafenacil applied at those rates had no negative season-long effect because observed early season injury was not manifested as a reduction in rice height 2 WAT or rough rice yield. Application of tiafenacil to crops directly adjacent to rice in its early vegetative stages of growth should be avoided because visual injury will occur. When off-target movement does occur, however, the affected rice should be expected to fully recover with no effect on growth or yield, assuming adequate growing conditions and agronomic/pest management are provided.

Waterhemp is a dioecious species with wide genetic diversity which has enabled it to evolve resistance to several commonly used herbicide groups in North America. Five field trials were established in Ontario to ascertain the biologically effective doses of diflufenican, a new Group 12 herbicide applied preemergence for control of multiple herbicide–resistant (MHR) waterhemp in corn. Based on regression analysis, the predicted diflufenican doses to elicit 50%, 80%, and 95% MHR waterhemp control were 99, 225, and 417 g ai ha−1, respectively, at 2 wk after application (WAA); 73, 169, and 314 g ai ha−1, respectively, at 4 WAA; and 76, 215, and — (meaning the effective dose was beyond the set of doses in this study) g ai ha−1, respectively, at 8 WAA. The predicted diflufenican doses that would cause a 50%, 80%, and 95% decreases in MHR waterhemp density were 42, 123, and — g ai ha−1; and MHR waterhemp biomass were 72, 167, and 310 g ai ha−1, respectively, at 8 WAA. Diflufenican applied preemergence at 150 g ai ha−1 controlled MHR waterhemp by 64%, 79%, and 73% at 2, 4, and 8 WAA, respectively. Isoxaflutole + atrazine applied preemergence at 105 + 1,060 g ai ha−1 controlled MHR waterhemp by 98%, 98%, and 97% at 2, 4, and 8 WAA, respectively; and S-metolachlor/mesotrione/bicyclopyrone/atrazine applied preemergence at 1,259/140/35/588 g ai ha−1 controlled MHR waterhemp by 100%, 100%, and 99% at 2, 4, and 8 WAA, respectively. Diflufenican applied preemergence reduced MHR waterhemp density and biomass by 83%; in contrast, isoxaflutole + atrazine and S-metolachlor/mesotrione/bicyclopyrone/atrazine reduced MHR waterhemp density and biomass by 99%. All treatments evaluated caused either no, or minimal, corn injury and resulted in corn yield that was similar with the weed-free control. Results indicate that diflufenican applied alone preemergence does not provide superior MHR waterhemp control over the commonly used herbicides isoxaflutole + atrazine or S-metolachlor/mesotrione/bicyclopyrone/atrazine; however, there is potential for using diflufenican as part of an integrated weed management strategy for the control of MHR waterhemp control in corn.

Mixing ammonium sulfate (AMS) can increase dicamba volatility. Therefore, AMS cannot be used with dicamba products in dicamba-resistant soybean. However, most dicamba products applied in corn are labeled to mix with AMS. The objectives of this study were to evaluate broadleaf weed control with dicamba (DiFlexx®) and dicamba/tembotrione (DiFlexx® DUO) applied alone or with AMS or AMS substitute and their effect on broadleaf weed density and biomass. Field experiments were conducted in Illinois, Missouri, and Nebraska in 2018 and 2019. In Illinois and Nebraska, mixing AMS + crop oil concentrate (COC) with dicamba applied at 1,120 g ae ha−1 increased the control of Palmer amaranth and waterhemp (Amaranthus species) from 78% to 92% and velvetleaf from 73% to 96% compared with dicamba applied alone 14 d after application (DAA); however, Missouri data showed no difference. Mixing AMS + COC with dicamba/tembotrione at 597 and 746 g ai ha−1 did not improve broadleaf weed control 14 DAA at any site compared with dicamba/tembotrione applied alone. Control of Amaranthus species was increased from 82% with dicamba applied at 840 g ae ha−1 to 96% when mixed with AMS + COC 28 DAA in Illinois; however, control was similar to dicamba applied at 1,120 g ae ha−1. Broadleaf weed control did not differ among dicamba or dicamba/tembotrione 28 and 56 DAA in Missouri and Nebraska. Broadleaf weed density decreased from 64 plants m−2 to 24 plants m−2 with dicamba at 1,120 g ae ha−1 with AMS + COC 14 DAA in Nebraska; however, no differences were observed in broadleaf weed density or biomass 56 DAA in any state. The results suggest that dicamba or dicamba/tembotrione can be applied without AMS or AMS substitute, especially at higher rates, without losing broadleaf weed control efficacy.

Technical limitations have restricted investigations of rhizosphere mineralogy. Various analytical techniques were applied to assess root-mineral associations and dynamics in natural soils under corn production. Soil samples were collected between four and five weeks after planting and included rhizospheric and non-rhizospheric soils, and undisturbed block samples containing corn root systems. Analytical techniques were applied and included; X-ray diffraction, optical microscope, SEM, EDXRA with SEM, transmission electron microscope (TEM), electron energy loss spectra with TEM, high-resolution transmission electron microscope (HRTEM) and microanalysis with HRTEM. The mineralogy of the rhizosphere differed from that of the bulk soil. Within the rhizosphere, minute platy particles which were mostly vermiculitic minerals, were particularly concentrated near or on root surfaces. These platy mineral particles were not attached to the entire area, but only to certain areas of root surfaces. Therefore, we report quantitative evidence for mineralogical changes in the rhizosphere in soil environments.

Much historical yield-monitor data is from fields where a uniform rate of nitrogen was applied. A new approach is proposed using this data to get site-specific nitrogen recommendations. Bayesian methods are used to estimate a linear plateau model where only the plateau is spatially varying. The model is then illustrated by using it to make site-specific nitrogen recommendations for corn production in Mississippi. The in-sample recommendations generated by this approach return an estimated $9/acre on the example field. The long-term goal is to combine this information with other information such as remote sensing measurements.

Soil fertility and disturbance can potentially modify weed dynamics in a corn–soybean rotation. Knowing how added mineral fertilizers and tillage influence weed populations in the long term can provide insight into weed community shifts, which may affect future weed management requirements. The first objective of this study was to evaluate the effect of 24 to 25 years of nitrogen and phosphorus fertilization treatments and tillage (moldboard vs. no-till) on weeds (density, biomass, and composition) before and after herbicide applications in 2016/corn and 2017/soybean. The second objective was to evaluate the effect of the same treatments 6 yr after no postemergence weed management in 2022/corn. Since the last evaluation performed in 2004, weed density, richness, and diversity increased, and more annual grasses were observed under both tillage regimes. No fertilization effect was observed on any weed variables, including composition, except for increased biomass when left to grow all season after crop planting. In managed plots, the density and biomass of annuals and perennials were generally higher in no-till, and discrepancies were highest for annual grass densities before herbicide application. Weed species richness and diversity based on counts were equivalent between tillage regimes, but total biomass was distributed between more species in no-till. Higher weed densities and concurrent weed biomass, observed in no-till, reduced crop yields in 2016/soybean only. The combination of low crop diversity and low use of residual herbicides during the trial potentially led to the observed species shifts and increased weed density.

The properties that might influence the sequestration of aflatoxin B1 (AfB1) were examined. Laser-diffraction, particle-size analysis (LDPSA) indicated that the particle size of the smectite influences the amount of AfB1 adsorbed. Effective adsorbent smectites disperse well under combined sodium hexametaphosphate solution and ultrasonic agitation. Particle size explained 66% of the variability for most of the samples investigated in an ‘as-received’ state. One effective adsorbent smectite was especially well aggregated and required additional physical dispersion, thus raising the correlation to 73%. Transmission electron microscope (TEM) images show typical smectites and reveal the very diverse morphology of smectites in bentonites. Thin, cloud-like smectite, in TEM images, related positively to AfB1-adsorption capacity. Particles that often fold and are usually ∼0.5 µm across seem to be optimal. The selection of criteria for evaluating these smectites provides a scientific basis for their selection to obtain reliable performance. Particle size is of particular importance as outlined below, and use of LDPSA makes it possible to perform the analysis efficiently and with precision.

Intensifying crop–fallow systems could address increased weed control costs, increased land or rental costs, reduced crop diversity, and degraded soil properties in water-limited environments. One strategy to intensify such systems could be the insertion of a short-season crop during fallow. But, how this strategy affects soils, crop production, and farm economics needs further research. Thus, we studied the impacts of replacing fallow in a winter wheat (Triticum aestivum L)–corn (Zea mays L.)–fallow system with a short-season spring crop [field pea (Pisum sativum L.)] on crop yields and economics from 2015 to 2019 and 5-yr cumulative effects on soil properties using an experiment in the west-central US Great Plains. After 5 yr, replacing fallow with field pea increased microbial biomass by 294 nmol g−1 and plant available water by 0.08 cm3 cm−3, and reduced bulk density by 0.1 g cm−3 and cone index by 0.73 MPa in the 0–5 cm depth. It had, however, no effect on other soil properties. Field pea yield averaged 2.24 Mg ha−1. Field pea reduced subsequent crop yield by 15–25% in two of three crops compared with fallow. However, economic analysis showed replacing fallow with field pea may improve net income by $144–303 ha−1, although income across the 5 yr differed by $65 ha−1 in favor of fallow. Replacing fallow in winter wheat–corn–fallow rotation with a short-season spring crop offers promise to improve some near-surface soil properties while increasing net economic return during fallow under the conditions of this study.

Delaying cover crop termination until cash crop planting (i.e., planting green) is an emerging no-till practice. Improved management recommendations are needed for optimizing weed suppression benefits while minimizing other pest, fertility, and crop management risks when planting green in corn production systems. In a 2-yr field experiment, we evaluated the interaction between cereal rye residue management tactics (standing residue, roll-crimping, roll-crimping with row cleaners) and herbicide programs (1-pass preemergence [PRE], 2-pass postemergence [POST]) when planting green on weed recruitment spatial patterns and corn performance compared to standard termination (14 d preplant [DPP]) and ryelage harvest (14 DPP) practices. In a 2-yr on-farm experiment, we evaluated corn performance in response to the same residue management tactics. Cereal rye biomass production varied significantly across years in on-station experiments, with average (4.9 Mg ha−1) and anomalous (9.9 Mg ha−1) levels observed in 2020 and 2021, respectively. In 2020, planting green with an integrated roll-crimper/row cleaner system resulted in greater intrarow weed density compared with planting green into standing cereal rye. Interrow weed density was lower when roll-crimping was employed compared to early termination (14 DPP). Planting green into standing cereal rye resulted in greater mean corn height (V5 stage) compared to other treatments, but corn population and yield did not differ. In 2021, few differences in weed recruitment patterns were observed, but corn population and yield were significantly lower in planting green treatments compared to early termination. In both years, late-season weed biomass was lower in two-pass POST programs compared to one-pass PRE programs. On-farm trials showed that planting green into standing residue increases corn height and can reduce corn populations, which may lead to reduce yields. Our results suggest that management recommendations for optimizing herbicide application timing should consider intrarow and interrow weed recruitment dynamics associated with residue management tactics needed to optimize corn performance.

Widespread occurrence of herbicide-resistant weeds and more variable weather conditions across the United States has made weed control in many crops more challenging. Preemergence (PRE) herbicides with soil residual activity have resurged as the foundation for early season weed control in many crops. Field experiments were conducted in Janesville and Lancaster, Wisconsin, in 2021 and 2022 (4 site-years) to evaluate the weed control efficacy of solo (single site of action [SOA]) and premix (two or more SOAs) PRE herbicides in conventional tillage corn. Treatments consisted of 18 PRE herbicides plus a nontreated check. At the Janesville-2021 site, S-metolachlor + bicyclopyrone + mesotrione, atrazine + S-metolachlor + bicyclopyrone + mesotrione, and clopyralid + acetochlor + mesotrione provided >72% giant ragweed control. At the Janesville-2022 site, none of the PRE herbicides evaluated provided >70% giant ragweed control due to the high giant ragweed density and the lack of timely rainfall. At the Lancaster-2021 site, atrazine, dicamba, and flumetsulam + clopyralid provided <45% waterhemp control, but the remaining treatments provided >90% control. At the Lancaster-2022 site, the efficacy of some PRE herbicides was reduced due to the high waterhemp density; however, most herbicides provided >75% control. At the Lancaster-2021 and Lancaster-2022 sites, only dicamba and S-metolachlor did not provide >75% common lambsquarters control. Group 15 PRE herbicides provided >75% control of giant foxtail. Across weed species, PRE herbicides with two (78%) and three (81%) SOAs provided greater weed control than PRE herbicides with a single SOA (68%), indicating that at least two SOA herbicides applied PRE result in better early season weed control. The efficacy of the PRE herbicide treatments evaluated herein varied according to the soil seedbank weed community composition and environmental conditions (i.e., rainfall following application), but the premixes were a more reliable option to improve early season weed control in conventional tillage corn.

Drill-interseeding cover crops into standing corn (V3 to V5 growth stage) provides opportunities for increasing functional diversity in cropping systems by facilitating use of cover crop mixtures that include grass, legume, and brassica species. Designing herbicide-based weed control programs that negotiate tradeoffs between crop protection and environmental goals when interseeding mixtures remains a major challenge. The objective of this study was to use greenhouse-based dose-response assays to describe the relative sensitivity of 12 cover crop species that differ in traits, including taxonomic group and seed mass, to chloroacetamide (acetochlor, dimethenamid, S-metolachlor; Group 15 herbicides as categorized by the Weed Science Society of America) and pyrazole (pyroxasulfone; Group 15) herbicides. Nonlinear models were fit, and effective doses were estimated (ED50) to compare relative sensitivities of cover crop species to each herbicide. Brassica species (winter canola, Dwarf Essex rape, daikon radish) were less sensitive than small-seeded legumes (medium red clover, crimson clover) and grasses (annual ryegrass) in response to each chloroacetamide, but similar in response to pyroxasulfone. Austrian winter pea, a large-seeded legume, was less sensitive to each herbicide compared to other legumes (crimson clover, medium red clover, hairy vetch). Cereal rye and triticale were less sensitive to dimethenamid, S-metolachlor, and pyroxasulfone compared to annual ryegrass, but similar sensitivities were observed for acetochlor. These results suggest that relative differences in sensitivity to chloroacetamide and pyrazole herbicides could be exploited when designing cover crop mixtures for interseeding. It is imperative, however, to use these findings in conjunction with field-based observations of cover crop injury potential in interseeded systems.

The development of an integrated weed management (IWM) strategy for control of multiple herbicide-resistant (MHR) waterhemp can provide field crop producers with a strategy to deplete the number of waterhemp seeds in the soil seedbank. Field experiments were established on two commercial farms in Ontario, Canada, with MHR waterhemp in 2017. The number of waterhemp seeds in the seedbank at the Cottam and Walpole Island sites prior to establishing the experiments was 413 and 40 million seeds ha−1, respectively. The goal of this 9-yr study is to document the depletion in the number of waterhemp seeds in the seedbank after Years 3, 6, and 9 (spring 2020, 2023, and 2026) and to identify management practices that can reduce the number of waterhemp seeds by 95% or more. Relative to the number of seeds in the soil seedbank when the experiment was initiated, at the Cottam site after 3 yr of this experiment, in the “control” treatment (continuous soybean seeded in rows spaced 75 apart, and sprayed with glyphosate) there was a numeric 31% increase in the number of waterhemp seeds in the seedbank; in contrast, in the three-crop rotation of corn/soybean/winter wheat (with or without a cover crop after winter wheat harvest), soybean seeded in rows spaced 37.5 cm apart, with herbicide applications using a total of eight different herbicide modes of action resulted in a 65% to 66% decrease in the number of waterhemp seeds in the soil seedbank. At the Walpole Island site after 3 yr of this experiment, the number of waterhemp seeds in the seedbank was not affected by the IWM programs evaluated. Results indicate that a diversified integrated waterhemp management program dramatically decreased the number of waterhemp seeds in the seedbank at one of two sites.