Eusebius’ much-discussed catalogue of ‘acknowledged’, ‘disputed’ and ‘spurious’ works (Historia Ecclesiastica 3.25) is a key passage in the history of New Testament canon formation, but it is often extracted from its literary context and consequently misunderstood. This passage is in fact a summary of conclusions that Eusebius has already reached in the contributions to apostolic biography with which he supplements the Book of Acts in HE 2.1–3.24. Biographical passages relating the conclusion of the apostolic lives of James, Peter, Paul and John are accompanied by statements about the texts they authored or authorised, or that have been falsely attributed to them. This biographical context for differentiating genuine works of prestigious figures from their pseudepigraphal counterparts has its roots in Greco-Roman literary culture, as exemplified in the Lives of the Philosophers of Diogenes Laertius. Eusebius’ crucial contribution to the formation of the New Testament canon is thus rooted not in exclusively Christian concerns but in the wider literary culture of Late Antiquity.