Development of exact doses (<100) of Strongyloides venezuelensis third-stage larvae in adult Wistar rats was insignificant (mean proportion of 0·076 of the dose at day 8, n = 16) compared with a homogonic strain of S. ratti (0·538, n = 6; 0·726, n = 6) and heterogonic S. ratti (0·681, n = 6). Newly-weaned Wistars allowed development of a mean proportion of S. venezuelensis of 0·298 (n = 4) compared with 0·013 (n = 4) of the same sample of larvae in adult hosts. Experiments with 75Se-labelled larvae established that S. venezuelensis effectively failed to migrate from skin to intestine in adult animals, while mean proportions of 0·141 (n = 5) and 0·138 (n = 4) of the label was found in the intestines of newly-weaned rats 72 h after skin application. Labelled larvae of homogonic S. ratti migrated equally well in both age groups of host (0·350 and 0·358 in 12- and 3-week-olds respectively). Adult S. venezuelensis transferred surgically to the intestines of previously uninfected full-grown Wistars survived over a 21-day period to the same extent as either strain of S. ratti. Resistance of Wistar rats to S. venezuelensis therefore appears to affect the migratory stage preferentially. S. venezuelensis developed better in mature PVG inbred rats (mean = 0·301, n = 20). Studies of S. ratti showed that infections of both strains initiated by exact (<100) doses in Wistar rats had decayed to insignificance between days 26 and 32. The rate of loss of adults of the heterogonic strain was significantly greater than that for the homogonic. The egg content of worms declined as infection progressed and rats were idiosyncratic in their influence on parasite reproduction from the earliest time of sampling (8 d). It was established that ‘autoinfection’ was an unlikely feature of the biology of homogonic S. ratti following the surgical transfer of 450 first-stage larvae to the intestines of 8 adult Wistar rats. No evidence of infection appeared in the guts of these animals 8 days post-transfer. The significance of these results in terms of the biology of Strongyloides spp. naturally occurring in the rat is discussed.

Infective larvae of Onchocerca lienalis and O.volvulus implanted subcutaneously within micropore chambers into laboratory hosts moulted to the fourth stage (L4) and underwent limited development and growth. Similar recoveries of O. lienalis L4 larvae in the range of 33–66% were obtained from chambers implanted into CBA and BALB/c strains of mice, jirds, and the natural bovine host. A relatively constant proportion of larvae survived up to 24 days post implantation and thereafter recoveries declined, although some worms were still alive after 96 days. Recoveries of O. volvulus L4 larvae from chambers given to normal or T-cell deprived mice were equivalent to one another and to those obtained with O. lienalis. Moulting of O. lienalis in chambers was observed on days 3 and 5, in close accordance with the timing of the third moult in cattle following systemic infection. Moulting of O. volvulus occurred between days 3–6. Morphological changes in developing larvae included a small but significant increase in length, a transient increase in width, and early development of the spicular primordia and genital tube. L4 larvae of O. lienalis, but not those of O. volvulus, exhibited 3 distinct caudal papillae not present on infective larvae.

Hymenolepis murissylvatici elicits a humoral response in serum and in the intestine of the mouse from which it is immunologically rejected. In serum, significant differences were recorded 3 days after reinfection, while in intestinal lavages it takes place from day 9 after reinfection. In serum the response is largely the result of IgG and IgM antibodies whereas in the intestine, IgA is the most abundant antibody. Although specific IgE could not be demonstrated in serum, it was present in intestinal lavages, although rather late (i.e. day 14 after reinfection). Treatment of young worms in vitro both with immune serum or intestinal lavages had no apparent effect on their viability. Immune serum produced a complement independent precipitation on the surface of the worms in vitro. This reaction did not affect viability or infectivity of the parasite, as demonstrated by the successful implantation of treated worms in recipient mice. The above-mentioned results, together with the knowledge that the Hymenolepis model has no tissue stages and causes no tissue damage, make it probable that further study of this model will reveal some specific intestinal immunological reactions.

Domestic chicks experimentally infected with Echinostoma caproni for 2 weeks showed a dilated ileum, unkempt feathers, watery diarrhoea, and weight loss. The ileum from infected and control chicks was fixed in 10% neutral buffered formalin, and prepared as 10 μm paraffin sections stained in haematoxylin and eosin, Papanicolau, periodic acid-Schiff, picro-ponceau, and alcian blue. The infected ileum showed atrophic villi, hypertrophied circular musculature with collagen-like fibres and haemorrhagic zones. The brush borders of epithelial cells and goblet cells were absent in the mucosa of the infected ileum. Worms in contact with host mucosa showed tissue plugs in the suckers. The cephalic spines of worms abraded the host mucosa.

This study investigates the level of helminthic infestation in better-cared-for dogs in a middle-class community in suburban Kingston. A canine zoographic study was conducted, and fresh faecal deposits were collected and analysed for helminth life-cycle stages. The survey indicated that 73% (n=93) of households in the study area owned one dog or more (mean=1·4). Resident's attitudes towards canine management suggested that the dog population was, in general, restricted to the residential estate, and most owners claimed to have dewormed their dogs at least as young animals. Of 141 faecal specimens, 58% contained eggs or larvae of one or more of eight helminths: Uncinaria stenocephala (26%), Ancylostoma sp. (23%), Trichuris vulpis (9%), Toxocara canis (8%), Spirocerca lupi (6%), Strongyloides sp. (6%), Apophallus sp. (4%) and taeniids (1%). There was a high level of multiple infection in the host animals, with approximately one fifth of the infected samples containing three or more helminth types. Infection intensity was apparently low, but some dogs harboured heavy worm loads.

Artificially prepared cow pats containing Ostertagia ostertagi eggs were deposited on two comparable grazing plots at weekly intervals during June and July 1987. Before deposition the cow pats, on the one plot, were inoculated with 0·250 g mycelial fragments per kg of faeces of the predacious fungus Arthrobotrys oligospora. On the other control plot comparable numbers of non-inoculated cow pats were placed at the same time. Subsequently, in July, two equal groups of calves were turned out one on each plot to graze for two months after which they were necropsied. The number of infective O. ostertagi larvae in inoculated cow pats was reduced by 42% and herbage larval infectivity around them by 50–71% as compared with the corresponding parameters in control pats and surrounding herbage. After a grazing period of two months the calves on the plot with inoculated cow pats had acquired worm burdens that were on average 37% lower than those of calves on the control plot. The lower degree of parasitism in these calves was also reflected in lower egg counts, lower serum pepsinogen levels and higher body weight.

Muellerius capillaris was the only species of nematode recovered from the lungs of young and adult sheep and goats of north-east Zaire. The prevalences were of 19% (sheep) and 32% (goats). The slug Atoxon pallens contained larvae in natural conditions and represents a potential intermediate host. The land snails Achatinidae (Achatina stuhlmanni and Limicolaria spp.) could not be experimentally infected with M. capillaris larvae.

Experimental Angiostrongylus costaricensis infection was carried out in inbred strains of mice (C57BL/6, BALB/c, DBA/2 and C3H/He). All strains became infected with this parasite. Marked differences in mortality and in worm burden were found among inbred strains of mice tested. A significant reduction was shown in worm length from mice compared to that from cotton rats.

An echinostome cercaria (? Cercaria spinifera La Valette, 1855) with 37 collar spines and paraoesophageal glands has been recorded for the first time in Britain from the gastropod Planorbarius corneus. The cercariae penetrate into and encyst in planarians. Observations made on cercariae during penetration indicate that the paraoesophageal glands are used to enter the body of the planarian and that the so-called penetration glands have some other function. Gastropod molluscs may also serve as second intermediate hosts, but there is evidence to indicate that anatomically similar cercariae from different host individuals vary in their second intermediate host preferences.

The present study investigated the suitability and importance of buffaloes, camels, sheep, goats and pigs in maintaining the life-cycle of Echinococcus granulosus in Aligarh, India. A total of 565 (36%) of 1556 buffaloes, 20 (2%) of 1208 goats, 5 (1%) of 559 pigs, 6 (6%) of 109 sheep and two of three camels were found to harbour hydatid cysts. The frequency distribution of the hydatid cysts in each intermediate host species was over-dispersed and in buffaloes cyst fertility increased with increasing cyst size. Of 2171, 95 and four buffalo, goat, and camel cysts examined 327 (15%), two (2%) and three cysts respectively were fertile. No pig or sheep cysts were found to contain protoscoleces. The unfenced buffalo abattoir and the large number of dogs allowed access to the abattoir coupled to the number of buffaloes slaughtered in comparison to the other potential hosts, indicates that the buffalo is the most significant host for maintaining the life-cycle of the parasite in this area of India. Applicable control measures for the region are suggested.

Five experimental grass paddocks were sequentially contaminated with fresh bovine faeces containing known numbers of eggs of predominantly Haemonchus and Cooperia spp. during the 1984/85 dry season (November to March). Faecal, herbage and soil samples were examined at regular intervals between November and June in order to determine the rate of development and mortality of infective larvae (L3) in faeces, the pattern of herbage infestation with L3 and the role of faeces and soil as reservoirs of L3 during the dry season and early rains. L3 first appeared in faeces approximately 4 days post contamination (PC) and peak counts were obtained 28, 14, 7 and 14 days PC in the paddocks contaminated in December, January, February and March, respectively. The counts initially declined linearly at the rate of approximately 7535 L3/week and 10 947 L3/week in P2 and P4 respectively, due primarily to mortality but later there was an accelerated fall in the counts as the surviving L3 moved out of the faecal pads onto herbage. The overall trend of faecal larval populations in each paddock was therefore distinctly curvilinear. Although large numbers of L3 were present inside dry faecal pads in most paddocks throughout the dry season, none migrated on to herbage at that time of the year. Translation of L3 to herbage was very rapid and occurred simultaneously in all the paddocks 24 hours following the first heavy rainfall in late March. Consequently peak herbage infestations in all paddocks were coincident and occurred a few days after commencement of larval migration. The closer to the end of the dry season the contaminations were carried out, the larger were the subsequent early rains rise and the peak herbage infestation and the longer this infestation survived on herbage. No L3 were recovered from soil throughout the study, which suggests that faecal pads were the sole reservoir of L3 during the dry season and hence the source of the early rains herbage infestation.

The generation of protective immunity by various stages in the life-cycle of Strongyloides ratti and the phases against which resistance is directed has been examined in murine strongyloidiasis. Mice were exposed to natural, complete infections, were treated with thiabendazole (which largely resembles the natural infection), were treated with cambendazole (which restricts infection to the larval stage), or infected directly by oral transfer of adult worms. Mice that were infected with infective larvae alone did not become resistant to infective larvae or the complete infection but were resistant to adult worms implanted directly into the gut. Mice exposed to adult worms alone were resistant to natural infections and adults worms implanted directly but were not resistant to infective larvae. On the other hand, mice that had received prior natural infections showed evidence of resistance to infective larvae, adult worms, and natural, complete infections. It is concluded that there is immunological cross-reactivity between infective larvae and adult worms but that under certain circumstances the infective larvae are able to evade the host's protective immune response.

Comparative ultrastructural studies have been made of newly excysted metacercariae and worms recovered from abnormal (rats) and normal definitive hosts (dogs). Worms from rats survived for prolonged periods without any growth or development in muscles of the host. They appeared to resemble metacercariae in general features, although ultrastructural observations revealed differences in the composition of tegumental granules and the development of the excretory bladder and caeca. Worms from dogs showed well-developed morphology in association with active energy metabolism and biosynthesis.

Histochemical tests were done on newly excysted metacercariae and worms recovered from an abnormal host (rat) and the definitive host (dog) for some oxidoreductases, phosphatases and glycosidases. The results demonstrate that rat worms have enzymatic distribution and intensities more similar to those of metacercariae than to adult worms from dogs. Ultracytochemical examination of acid and alkaline phosphatase and Mg-ATPase activity was also carried out. Acid phosphatase activity occurred exceptionally in the excretory bladder and caeca of dog worms. No activity was observed in rat worms except for lysosomal granules in the tegument. Alkaline phosphatase activity was exhibited in the excretory bladder in both dog and rat worms. Mg-ATPase activity occurred in the tegument and parenchymal cells in dog worms and in the excretory bladder in rat worms. In metacercariae, little or no reaction for these enzymes was present except for Mg-ATPase activity on the excretory ducts. These observations, together with the histochemical results, indicate that metabolic activity in rat worms is higher than in metacercariae although it is strongly reduced compared with dog worms.

During one year 1249 male cattle were examined for Mammomonogamus laryngeus infections in the slaughterhouse at Kandy, Sri Lanka. The overall prevalence was 40% with only light monthly variations (34 to 52%). The infection rate was highest (47%) in 2 to 2·5 year old animals. In infected animals an average of 6·4 parasite pairs was found with higher numbers in older animals. The majority of worms were located on the posterior side of the epiglottis. Lesions observed were mucosal plugs at the site where the parasites were attached to the mucosa and moderate to severe erosions and ulcers in other zones.

The surface structure of adult Haplorchis pumilio from experimentally infected hamsters was studied in detail using scanning electron microscopy. Almost the entire surface of the worm was covered with scale-like spines regularly arranged in transverse rows. Most spines were pectinate. The size of the spines and the number of the teeth in each individual were largest in the spines around the middle region of the worm. The area surrounding the excretory pore was free of spines displaying a slightly wrinkled appearance. Many ciliated papillae were present on the anterior two-thirds of the body. The papillae on the oral sucker were more abundant than those elsewhere. The body papillae were distributed in a bilaterally symmetrical arrangement, appearing in groups, pairs or singly between the spines.