Emotion recognition, the ability to interpret others’ emotional expressions and infer mental states, is crucial for caregiver–child interactions. The ability to accurately recognize infant emotions may facilitate attuned and responsive caregiving. Across two studies, we validate a novel measure to assess the recognition of infants’ emotions (Reading the Mind in Infant Eyes Test [RMIET]) and investigate how this ability relates to observed caregiving. Study 1 examined item-level performance in 55 infant mental health experts and 100 undergraduate students. Study 2 examined RMIET scores in 133 pregnant people and their later caregiving when their children were 18-month-old. In Study 1, agreement was high among both mental health experts (ICC = .82) and undergraduate students (ICC = .93), providing evidence of the content validity of the RMIET. In Study 2, scores assessing the recognition of adult and infant emotions were positively correlated (r = .22, p = .012). After accounting for covariates, RMIET scores were statistically significantly associated with higher sensitivity and warmth and lower negative regard. Taken together, these studies provide preliminary evidence of content and predictive validity for the RMIET.