Mobilising indigenous and local knowledge systems has the potential to make their critical knowledge about landscapes and biodiversity meaningful as evidence in conservation and governance. Collaborative approaches to conservation must be equitable and just to be effective in the long term. The Multiple Evidence Base (MEB) is an inclusive approach to combining diverse sources of evidence. We review uptake of the MEB approach and its application in science and policy–practice processes and present three cases using this approach. These demonstrate synergies between indigenous and local knowledge and conservation science and how cross-fertilisation leads to stronger partnerships and better outcomes. However, this depends on recognition of the complementarity and respect for the integrity of diverse knowledge systems. The cases also illustrate ways to create conditions for dialogue and navigating power inequalities and cultural differences in knowledge partnerships. Ultimately, the MEB approach contributes to a conceptual mindshift to mobilise all useful knowledge for maintaining the life-supporting ecosystems on Earth.