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Effect of dehydroleucodine (DhL) on the acrosome reaction in sperm of Chinchilla lanigera: signalling pathways involved

Published online by Cambridge University Press:  19 November 2024

Maria Fernanda Medina ,
Ana Pucci ,
Franco José Pucci ,
Marta Ines Bühler  and
María Cecilia Gramajo-Bühler Open the ORCID record for María Cecilia Gramajo-Bühler [Opens in a new window]
Maria Fernanda Medina
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina
Ana Pucci
Affiliation:
Facultad de Ciencias Naturales, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina
Franco José Pucci
Affiliation:
Miguel Lillo Foundation, Animal Morphology Institute (AMI), San Miguel de Tucumán, Tucumán, Argentina
Marta Ines Bühler
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina
María Cecilia Gramajo-Bühler*
Affiliation:
Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina Department of Developmental Biology, Higher Institute for Biological Research, CONICET, San Miguel de Tucumán, Tucumán, Argentina
*
Corresponding author: María Cecilia Gramajo-Bühler; Email: [email protected]
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Abstract

The secondary metabolites of several plant species, particularly sesquiterpenic lactones (SLs) have been studied by different research groups for over 30 years. This group of metabolites presents numerous biological activities such as antibacterial, antiviral, antiulcer, cell proliferation inhibitor, and oocyte activator with participation in exocytosis processes. This study aims to assess some sperm parameters in epididymal gametes of Chichilla lanigera exposed to increasing concentrations (0 to 2 mM) of DhL for various incubation times from 10 to 40 minutes. We determined the participation of different cell signalling pathways in the induced acrosome reaction. Our results showed an alteration in the progressive motility pattern and cell viability depending on DhL concentration and exposure time of gametes. When analyzing acrosomal status, higher percentages than the negative control were obtained in all tested doses. Both isolated and joint inhibition tests of PKA and phospholipases (PLC and PLA2) showed a greater participation of PI-PLC. This is the first report concerning the effects of this lactone on the medium of sperm incubation. Consequently, further studies will be necessary to determine the molecular implications of this lactone on the fertilizing potential of the sperm.

Keywords

acrosome reactiondehydroleucodineepididymal spermatozoasesquiterpene lactonessperm quality
Type
Research Article
Information
Zygote , Volume 32 , Issue 6 , December 2024 , pp. 446 - 451
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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