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Seed set by artificial pollination and seed storage under cryogenic, freezer and dry conditions in the medicinal plant Uncaria rhynchophylla

Published online by Cambridge University Press:  15 November 2024

Keita Endoh Open the ORCID record for Keita Endoh [Opens in a new window] ,
Ken-ichi Konagaya Open the ORCID record for Ken-ichi Konagaya [Opens in a new window] ,
Michinari Matsushita Open the ORCID record for Michinari Matsushita [Opens in a new window]  and
Toru Taniguchi Open the ORCID record for Toru Taniguchi [Opens in a new window]
Keita Endoh
Affiliation:
Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki 319-1301, Japan
Ken-ichi Konagaya
Affiliation:
Forest Bio-Research Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki 319-1301, Japan
Michinari Matsushita
Affiliation:
Forest Tree Breeding Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki 319-1301, Japan
Toru Taniguchi*
Affiliation:
Forest Bio-Research Center, Forestry and Forest Products Research Institute, Hitachi, Ibaraki 319-1301, Japan
*
Corresponding author: Toru Taniguchi; Email: [email protected]
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Abstract

Uncaria rhynchophylla (Rubiaceae), a woody liana with significant medicinal value, has been used as a traditional Japanese and Chinese medicine. While effective seed production is required for breeding and efficient seedling production, the physiology of sexual reproduction remains largely unknown in this species. Therefore, we first observed the flowering behaviour, and next attempted artificial pollination using flowering individuals in a greenhouse. In this study it became clear that this species sets seeds by allogamy, but not by autogamy. The obtained seeds showed about a 90% germination rate. We also examined seed desiccation tolerance and storage conditions which are important to preserve the genetic resources. Seeds of this species were found to have a characteristic of the orthodox type, having high desiccation tolerance. Seeds after 6 months of storage at + 22, −20 and −160°C showed comparable germination rates to the seeds before storage.

Keywords

orthodox seedpharmaceutical plantsecondary pollen presentationseed storageUncaria hook
Type
Research Article
Information
Plant Genetic Resources , Volume 23 , Issue 2 , April 2025 , pp. 100 - 105
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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Footnotes

*

These authors contributed equally to this study.

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