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Ex-situ evaluation of curry leaf (Murraya koenigii (L.) Spreng) germplasm to unravel the genetic diversity of leaf essential oils

Published online by Cambridge University Press:  20 December 2024

Borehalli Rangaswamy Raghu Open the ORCID record for Borehalli Rangaswamy Raghu [Opens in a new window] ,
Kodthalu Seetharamaiah Shivashankara ,
Priyanka Mahadappa ,
Ankanahalli Narayanashetty Lokesha ,
Kebbahalli Shivashankarappa Nandini ,
Vala Keshava Rao ,
H.C. Prasanna  and
Sudip Kumar Dutta Open the ORCID record for Sudip Kumar Dutta [Opens in a new window]
Borehalli Rangaswamy Raghu*
Affiliation:
Indian Institute of Horticultural Research (ICAR-IIHR), Hesaraghatta, Bengaluru, 560089, India
Kodthalu Seetharamaiah Shivashankara
Affiliation:
Indian Institute of Horticultural Research (ICAR-IIHR), Hesaraghatta, Bengaluru, 560089, India
Priyanka Mahadappa
Affiliation:
Indian Veterinary Research Institute (ICAR-IVRI), Regional Campus, Hebbal, Bengaluru, 560024, India
Ankanahalli Narayanashetty Lokesha
Affiliation:
Indian Institute of Horticultural Research (ICAR-IIHR), Hesaraghatta, Bengaluru, 560089, India
Kebbahalli Shivashankarappa Nandini
Affiliation:
Indian Institute of Horticultural Research (ICAR-IIHR), Hesaraghatta, Bengaluru, 560089, India
Vala Keshava Rao
Affiliation:
Indian Institute of Horticultural Research (ICAR-IIHR), Hesaraghatta, Bengaluru, 560089, India
H.C. Prasanna
Affiliation:
Indian Institute of Horticultural Research (ICAR-IIHR), Hesaraghatta, Bengaluru, 560089, India
Sudip Kumar Dutta
Affiliation:
ICAR Research Complex for NEH Region, Sikkim Centre, Gangtok, Sikkim, 737 102, India
*
Corresponding author: Borehalli Rangaswamy Raghu; Email: [email protected]
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Abstract

The present study was conducted with the objective of studying the genetic diversity of essential oils (EOs) in curry leaf (CL) ex-situ. Chemometric methods and pattern analysis were employed to assess the genetic diversity of EOs and to characterise diverse sets of CL germplasm into different chemotypes. The study revealed a huge genetic diversity for EO yield and its composition among the tested genotypes. Cultivated types had significantly higher EO yields and showed a greater degree of genetic divergence compared to wild types. In total, 80 different compounds were identified in the EOs of CL and classified into major (6) and minor (74) compounds. The major compounds α-pinene, γ-terpinene, and α-selinene and 14 minor compounds were highly variable among the tested genotypes. They may play an important role in the formation of different chemotypes. Other important compounds, such as trans-caryophyllene and α-humulene, were more widely distributed among the tested genotypes and indicated their predominant occurrence in the EOs of CL. Some major compounds, such as valencene and γ-terpinene, showed a significant regional correlation, indicating the role of geographic factors in the evolution of different chemotypes. Furthermore, some compounds such as α-pinene, bornyl acetate, and camphene had significantly higher concentrations in wild types compared to cultivated types, indicating the influence of domestication through human selection on the composition of EOs in CL. A total of 4 major chemotypes were characterised, of which three new chemotypes are being reported for the first time in CL.

Keywords

chemometric analysischemotypescultivated typesgermplasmwild types
Type
Research Article
Information
Plant Genetic Resources , First View , pp. 1 - 15
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany

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References

Adams, RP (eds) (1995) Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy, 2nd Edn. Carol Stream, IL: USA: Allured Publishing Corporation.Google Scholar
Angioni, A, Barra, A, Coroneo, V, Dessi, S and Cabras, P (2006) Chemical composition, seasonal variability, and antifungal activity of Lavandula stoechas L. ssp. stoechas essential oils from stem/leaves and flowers. Journal of Agricultural and Food Chemistry 54, 4364–4370.CrossRefGoogle ScholarPubMed
Clevenger, JF (1928) Apparatus for the determination of volatile oil. Journal of the American Pharmaceutical Association 17, 346–349.Google Scholar
Dastur, JF (ed.) (1970) Medicinal Plants of India and Pakistan, 3rd Edn. Bombay: DB Taraporevala Sons & Co. Private Ltd.Google Scholar
Deshmukh, SK, Jain, PC and Agarwal, SC (1986) Antimicrobial activity of the essential oil of the leaves of Murraya koenigii (Linn.) Spreng (Indian curry leaf). Fitoterapia 57, 295–297.Google Scholar
Drury, HC (ed.) (1978) The Useful Plants of India, 2nd Edn. London: William H Allen & Co.Google Scholar
Ganesan, P, Phaiphan, A, Murugan, Y and Baharin, BS (2013) Comparative study of bioactive compounds in curry and coriander leaves: an update. Journal of Chemical and Pharmaceutical Research 5, 590–594.Google Scholar
Goutam, MP and Purohit, RM (1974) Antimicrobial activity of essential oils of the leaves of Murraya koenigii (L.) Spreng. (Indian curry leaf). Indian Journal of Pharmacology 36, 11.Google Scholar
Igara, C, Omoboyowa, D, Ahuchaogu, A, Orji, N and Ndukwe, M (2016) Phytochemical and nutritional profile of Murraya Koenigii (Linn) Spreng leaf. Journal of Pharmacognosy and Phytochemistry 5, 7–9.Google Scholar
Joseph, S and Peter, KV (1985) Curry leaf (Murraya koenigii), perennial nutritious leafy vegetable. Economic Botany 39, 68–73.CrossRefGoogle Scholar
Kamila, PK, Ray, A, Jena, S, Sahoo, A, Kar, SK, Nayak, S and Panda, PC (2021) Intraspecific variability in yield and chemical composition of essential oil of the endemic species Hypericum gaitii from different natural habitats of Eastern India. Plant Biosystems 156, 1167–1176.CrossRefGoogle Scholar
Khatoon, J, Verma, A, Chacko, N and Sheikh, S (2011) Utilisation of dehydrated curry leaves in different food products. Indian Journal of Natural Products and Resources 2, 508–511.Google Scholar
Kovats, E (1965) Gas chromatographic characterisation of organic substances in the retention index system. Advances in Chromatography 1, 229–247.Google Scholar
Macleod, AJ and Pieris, NM (1982) Analysis of the volatile essential oils of Murraya koenigii and Pandanus latifolius. Phytochemistry 21, 1653–1657.CrossRefGoogle Scholar
Mallavarapu, GR, Ramesh, S, Syamasundar, KV and Chandrasekhara, RS (1999) Composition of Indian curry leaf oil. Journal of Essential Oil Research 11, 176–178.CrossRefGoogle Scholar
Mallavarapu, GR, Rao, L and Ramesh, S (2000) Volatile constituents of the leaf and fruit oils of Murraya koenigii Spreng. Journal of Essential Oil Research 12, 766–768.CrossRefGoogle Scholar
Masotti, V, Juteau, F, Bessière, JM and Viano, J (2003) Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities. Journal of Agricultural and Food Chemistry 51, 7115–7121.CrossRefGoogle ScholarPubMed
Mohan, RS (2012) Curry leaf campaign. Spice India 25, 10–12.Google Scholar
Morshedloo, MR, Ebadi, A, Maggi, F, Fattahi, R, Yazdani, D and Jafari, M (2015) Chemical characterisation of the essential oil compositions from Iranian populations of Hypericum perforatum L. Industrial Crops and Products 76, 565–573.CrossRefGoogle Scholar
Onayade, OA and Adebajo, AC (2000) Composition of the leaf volatile oil of Murraya koenigii growing in Nigeria. Journal of Herbs, Spices & Medicinal Plants 7, 59–66.CrossRefGoogle Scholar
Pang, Z, Chong, J, Zhou, G, de Lima Morais, DA, Chang, L, Barrette, M, Gauthier, C, Jacques, P, Li, S and Xia, J (2021) Metaboanalyst 5.0: narrowing the gap between raw spectra and functional insights. Nucleic Acids Research 49, 388–396.CrossRefGoogle Scholar
Pathak, N, Yadav, TD, Jha, AN, Vasudevan, P and Pathak, N (1997) Contact and fumigant action of volatile oil of Murraya koenigii against Callosobruchus chinensis. Indian Journal of Entomology 59, 198–202.Google Scholar
Philip, J, Peter, KV and Gopalakrishnan, PC (1981) Curry leaf a mineral packed vegetable. Indian Horticulture 25, 2–3.Google Scholar
Poornima, KN, Nandini, KS and Raghu, BR (2022) Curry leaf a medicinal boon. Agriculture world 8, 24–27.Google Scholar
Prakash, V and Natarajan, CP (1974) Studies on curry leaf (Murraya koenigii L.). Journal of Food Science and Technology 11, 285–286.Google Scholar
Priya, RM, Blessed, BP and Nija, S (2013) Chemical composition, antibacterial and antioxidant profile of essential oil from Murraya koenigii (L.) leaves. Avicenna Journal of Phytomedicine 4, 200–214.Google Scholar
Raghu, BR (2020) Diversity and distribution of curry leaf in India. Journal of Horticultural Sciences 15, 1–8.CrossRefGoogle Scholar
Raghu, BR (2023) Curry leaf. In Dubey, RK and Singh, J (eds), Production Technology of Underexploited Vegetable Crops. New Delhi: Kalyani Publishers, pp. 72–85.Google Scholar
Raghu, BR and Nandini, KS (2021) Standardisation of seed propagation method in curry leaf. In International Conference on Vegetable Research and Innovations for Nutrition, Entrepreneurship and Environment (ICVEG-21), ICAR-IIVR, Varanasi (UP), India, 14–16th December 2021, pp. 445–446.Google Scholar
Raghu, BR, Aghora, TS and Dhananjaya, MV (2020) Genetic improvement of curry leaf in India: challenges and future prospects. Indian Horticulture 65, 127–130.Google Scholar
Raghu, BR, Aghora, TS and Dhananjaya, MV (2022) Curry leaf Improvement in India. In Compendium for winter School on “underexploited vegetables: unexplored treasure trove for food, nutritional and economic security, ICAR-IIVR, Varanasi (UP), India, 02–11 February, 2022, pp. 183–186.Google Scholar
Raina, VK, Lal, RK, Tripathi, S, Khan, M, Syamasundar, KV and Srivastava, SK (2002) Essential oil composition of genetically diverse stocks of Murraya koenigii. Flavour and Fragrance Journal 17, 144–146.CrossRefGoogle Scholar
Ram, P, Kumar, B, Naqvi, AA, Verma, RS and Patra, NK (2005) Post-harvest storage effect on quality and quantity of rose-scented geranium (Pelargonium sp. cv. Bourbon) oil in Uttaranchal. Flavour and Fragrance Journal 20, 666–668.CrossRefGoogle Scholar
Rana, VS, Juyal, JP, Rashmi, and Blazquez, MA (2004) Chemical constituents of the volatile oil of Murraya koenigii leaves. International Journal of Aromatherapy 14, 23–25.CrossRefGoogle Scholar
Rao, BRR, Rajput, DK and Mallavarapu, GR (2011a) Chemotype categorisation of curry leaf plants {Murraya koenigii (L.) Spreng.}. Journal of Essential Oil-Bearing Plants 14, 1–10.CrossRefGoogle Scholar
Rao, BRR, Rajput, DK and Mallavarapu, GR (2011b) Chemical diversity in curry leaf (Murraya koenigii) essential oils. Food Chemistry 126, 989–994.Google Scholar
Ray, A, Jena, S, Dash, B, Kar, B, Halder, T, Chatterjee, T, Ghosh, B, Panda, PC, Nayak, S and Mahapatra, N (2018) Chemical diversity, antioxidant and antimicrobial activities of the essential oils from Indian populations of Hedychium coronarium Koen. Industrial Crops and Products 112, 353–362.CrossRefGoogle Scholar
Ray, A, Jena, S, Haldar, T, Sahoo, A, Kar, B, Patnaik, J, Ghosh, B, Panda, PC, Mahapatra, N and Nayak, S (2019) Population genetic structure and diversity analysis in Hedychium coronarium populations using morphological, phytochemical and molecular markers. Industrial Crops and Products 32, 118–133.CrossRefGoogle Scholar
Sahoo, A, Kar, B, Jena, S, Dash, B, Ray, A, Sahoo, S and Nayak, S (2019) Qualitative and quantitative evaluation of rhizome essential oil of eight different cultivars of Curcuma longa L. (Turmeric). Journal of Essential Oil-Bearing Plants 22, 239–247.CrossRefGoogle Scholar
Sahoo, C, Champati, BB, Dash, B, Jena, S, Ray, A, Panda, PC, Nayak, S and Sahoo, A (2022) Volatile profiling of Magnolia champaca accessions by gas chromatography mass spectrometry coupled with chemometrics. Molecules 27, 7302.CrossRefGoogle ScholarPubMed
Salgotra, RK and Chauhan, BS (2023) Genetic diversity, conservation, and utilisation of plant genetic resources. Genes 14, 174.CrossRefGoogle ScholarPubMed
Santhanakrishnan, VP, Shoba, N, Varun, E, Mohankumar, S and Raveendran, M (2023) Aromatic profiling of Murraya koenigii leaves by Thermal Desorption Gas chromatography-Mass Spectroscopy (TD-GC-MS). Heliyon 9, e17832.Google Scholar
Senthilkumar, A, Gopalakrishnan, B, Jayaraman, M and Venkatesalu, V (2014) Chemical composition and antibacterial activity of essential oil from the leaves of Murraya koenigii (L.) Spreng. Journal of Experimental Sciences 5, 1–4.Google Scholar
Shamsheer, B, Riaz, N, Yousaf, Z, Hyder, S, Aftab, A, Iqbal, R, Rahman, MH, Al-Ashkar, IF, Almutairi, K and El Sabagh, A (2022) Genetic diversity analysis for wild and cultivated accessions of Cymbopogon citratus (D.C.) Stapf using phytochemical and molecular markers. PeerJ 10, e13505.CrossRefGoogle Scholar
Sukkaew, S, Pripdeevech, P, Thongpoon, C, Machan, T and Wongchuphan, R (2014) Volatile constituents of Murraya koenigii fresh leaves using headspace solid phase microextraction – gas chromatography – mass spectrometry. Natural Product Communications 9, 1783–1786.CrossRefGoogle ScholarPubMed
Syamasundar, KV, Srinivasulu, B, Ananda, PLG, Ramesh, S and Rao, RR (2012) Chemo variations of wild curry leaf (Murraya koenigii Spreng.) from Western Ghats of Indian. Journal of Pharmacognosy 3, 126–130.Google Scholar
Verghese, J (1989) Indian curry leaf. Perfumer & Flavorist 14, 69–70.Google Scholar
Verma, RS, Verma, RK, Chauhan, A and Yadav, AK (2009) Changes in the essential oil content and composition of Eucalyptus citriodora hook during leaf ontogeny and leaf storage. India Perfumer 53, 22–25.Google Scholar
Verma, RS, Rahman, L, Verma, RK, Chanotiya, CS, Chauhan, A, Yadav, A, Yadav, AK and Singh, A (2010) Changes in the essential oil content and composition of Origanum vulgare L. during annual growth from Kumaon Himalaya. Current Science 98, 1010–1012.Google Scholar
Verma, RS, Padalia, RC, Arya, V and Chauhan, A (2012) Aroma profiles of the curry leaf, Murraya koenigii (L.) Spreng. Chemotypes variability in north India during the year. Industrial Crops and Products 36, 343–348.CrossRefGoogle Scholar
Verma, RS, Chauhan, A, Padalia, RC, Jat, SK, Thul, S and Sundaresan, V (2013) Phytochemical diversity of Murraya koenigii (L.) Spreng. from western Himalaya. Chemistry & Biodiversity 10, 628–641.CrossRefGoogle ScholarPubMed
Vyas, VG, Kandoliya, UK, Vidhani, SI, Parmar, HJ, Bhalani, VM and Golakiya, BA (2015) Heavy metal deposition and phytochemical characterisation of curry leaves (Murraya koenigii). International Journal of Current Microbiology and Applied Sciences 4, 839–843.Google Scholar
Zhang, DY, Yao, XH, Duan, MH, Wei, FY, Wu, GH and Li, L (2015) Variation of essential oil content and antioxidant activity of Lonicera species in different sites of China. Industrial Crops and Products 77, 772–779.CrossRefGoogle Scholar
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