Hostname: page-component-669899f699-b58lm Total loading time: 0 Render date: 2025-05-02T20:51:04.799Z Has data issue: false hasContentIssue false
  • English
  • Français

Nanoscale Silicon Microcavity Optical Sensors for Biological Applications

Published online by Cambridge University Press:  17 March 2011

Selena Chan ,
Scott R. Horner ,
Benjamin L. Miller  and
Philippe M. Fauchet
Selena Chan
Affiliation:
University of Rochester, Center for Future Health, Rochester, NY 14627, U.S.A.
Scott R. Horner
Affiliation:
Department of Chemistry, Rochester, NY 14627, U.S.A
Benjamin L. Miller
Affiliation:
Department of Chemistry, Rochester, NY 14627, U.S.A
Philippe M. Fauchet
Affiliation:
Department of Electrical and Computer Engineering, Rochester, NY 14627, U.S.A
Get access

Abstract

The large surface area of porous silicon provides numerous sites for many potential species to attach, which makes it an ideal host for sensing applications. The average pore size can be easily adjusted to accommodate either small or large molecular species. When porous silicon is fabricated into a structure consisting of two high reflectivity multilayer mirrors separated by an active layer, a microcavity is formed. Multiple narrow and visible luminescence peaks are observed with a full width at half maximum value of 3 nm. The position of these peaks is extremely sensitive to small changes in refractive index, such as that obtained when a biological object is attached to the large internal surface of porous silicon. We demonstrate the usefulness of this microcavity resonator structure as a DNA optical biosensor which displays appropriate sensitivity, selectivity, and response speed. A probing strand of DNA is initially immobilized in the porous silicon matrix, and then subsequently exposed to its sensing complementary DNA strand. Red-shifts in the luminescence spectra are observed and detected for various DNA concentrations. The spectral shifts confirm successful recognition and binding of DNA molecules within the porous structure. Detailed device fabrication procedures and the results of extensive testing will be presented. The detection scheme has also been extended to include the detection of viral DNA, proteins, and potentially bacteria. This work will lead to the development of a “smart bandage”, where the detection of bacteria or viruses can be diagnosed and an antibiotic treatment can be recommended.

Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 638: Symposium F – Microcrystaline & Nanocrystalline Semiconductors-2000 , 2000 , F10.4.1
Copyright
Copyright © Materials Research Society 2001

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

References

1. Canham, L. T., Appl. Phys. Lett., 57, 1046 (1990).Google Scholar
2. Berger, M. G., Thonissen, M., Arens-Fischer, R., Munder, H., Luth, H., Arntzen, M., and Theiβ, W., Thin Solid Films, 255, 313 (1995).Google Scholar
3. Berger, M. G., Dieker, C., Thonissen, M., Vescan, L., Luth, H., Munder, H., Wernke, M., and Grosse, P., J. Phys. D: Appl. Phys., 27, 1333 (1994).Google Scholar
4. Frohnhoff, S. and Berger, M. G., Adv. Mater., 6, 963 (1994).Google Scholar
5. Chan, S. and Fauchet, P. M., Appl. Phys. Lett., 75, 274 (1999).Google Scholar
6. Chan, S., Fauchet, P. M., Li, Y., and Rothberg, L. J., Proc. SPIE, 3912, 23 (2000).Google Scholar
7. Theβ, W., “Optical properties of porous silicon,” Surface Science Reports, 29, pp. 91–192 (1997).Google Scholar
8. Lehninger, A. L., Nelson, D. L., and Cox, M. M., Principles of Biochemistry, Worth Publishers, New York, 1993.Google Scholar
9. Isola, N., Stokes, D. L., and Vo-Dinh, T., Anal. Chem., 70, 1352 (1998).Google Scholar
10. Janshoff, A., Dancil, K -P. S., Steinem, C., Greiner, D. P., Lin, V. S. -Y., Gurtner, C., Motesharei, K., Sailor, M. J., and Ghadiri, M. R., J. Am. Chem. Soc., 120, 12108 (1998).Google Scholar
11. Vo-Dinh, T., Alarie, J. P., Isola, N., Landis, D., Wintenberg, A. L., and Ericson, M. N., Anal. Chem., 71, 358 (1999).Google Scholar
12. Chan, S., Fauchet, P. M., Li, Y., Rothberg, L. J., and Miller, B. L., Phys. Stat. Sol. A, 182, 541 (2000).Google Scholar
13. Chan, S., Li, Y., Rothberg, L. J., Miller, B. L., and Fauchet, P. M., to be published in Physica E, (2001).Google Scholar
14. Hershey, A. D., The Bacteriophage Lambda The Cold Spring Harbor Laboratory, New York, USA (1971).Google Scholar
15. Lin, V. S. -Y., Motesharei, K., Dancil, K. -P. S., Sailor, M. J., and Ghadiri, M. R., Science, 278, 840 (1997).Google Scholar
16. Lehninger, A. L., Nelson, D. L., and Cox, M. M., Principles of Biochemistry 2nd Edition, Worth Publishers, New York (1993).Google Scholar
17. Hubbard, R. D., Horner, S. R., and Miller, B. L., to be published.Google Scholar