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Genomic characterization of a new species of Pseudoparacreptotrema (Digenea: Allocreadiidae) from Puerto Rico, with comments on the biogeography of the genus

Published online by Cambridge University Press:  18 November 2024

G.M. Torres-Nieves ,
D.D. López-Hernández  and
S.A. Locke Open the ORCID record for S.A. Locke [Opens in a new window]
G.M. Torres-Nieves
Affiliation:
Departamento de Biología, Recinto Universitario de Mayagüez, Universidad de Puerto Rico, Call Box 9000, Mayagüez 00681-9000, Puerto Rico
D.D. López-Hernández
Affiliation:
Departamento de Biología, Recinto Universitario de Mayagüez, Universidad de Puerto Rico, Call Box 9000, Mayagüez 00681-9000, Puerto Rico Department of Parasitology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
S.A. Locke*
Affiliation:
Departamento de Biología, Recinto Universitario de Mayagüez, Universidad de Puerto Rico, Call Box 9000, Mayagüez 00681-9000, Puerto Rico
*
Corresponding author: S.A. Locke; Email: [email protected]
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Abstract

In this study, we describe a new species of Pseudoparacreptotrema (Allocreadiidae) from the mugilid Dajaus monticola collected in western Puerto Rico, where no allocreadiid has previously been reported, bringing the number of species in this genus to seven (five in D. monticola, two in Profundulus spp.). The new parasite species is distinguished from congeners by its overall size, oral-to-ventral sucker size ratio, pharynx size, cirrus sac, and oral lobe morphology, and by 0.64%–3.45% divergence in a 1019-bp alignment of 28S. We build on prior suggestions that the current concept of P. agonostomi likely includes multiple species and provide the first mitochondrial data (whole mitochondrial genome) as well as the complete nuclear rDNA array from Pseudoparacreptotrema to facilitate future phylogenetic work. Within the Allocreadiidae, phylogenetic analysis of mitochondrial genomes and 28S provides conflicting topologies for the placement of Pseudoparacreptotrema and Allocreadium. The 28S phylogeny of six species of Pseudoparacreptotrema resembles that of four lineages of D. monticola in that in both host and parasite, Pacific coastal lineages branch earliest, and a Caribbean lineage is more recently evolved.

Keywords

mountain mullethelminthnext generation sequencingCaribbeanCentral America
Type
Research Paper
Information
Journal of Helminthology , Volume 98 , 2024 , e72
Copyright
© The Author(s), 2024. Published by Cambridge University Press

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