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First integrative assessment of Ochetosoma heterocoelium (Travassos, 1921) (Digenea: Ochetosomatidae) parasite of Bothrops moojeni Hoge, 1966 (Reptilia: Viperidae) from Brazil

Published online by Cambridge University Press:  24 April 2025

E. Emmerich Open the ORCID record for E. Emmerich [Opens in a new window] ,
E. Alcantara ,
L. Úngari ,
L. O’Dwyer ,
G. Pérez-Ponce de León  and
R. Silva
E. Emmerich*
Affiliation:
São Paulo State University (UNESP), Institute of Biosciences, Section of Parasitology, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
E. Alcantara
Affiliation:
São Paulo State University (UNESP), Institute of Biosciences, Section of Parasitology, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
L. Úngari
Affiliation:
São Paulo State University (UNESP), Institute of Biosciences, Section of Parasitology, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
L. O’Dwyer
Affiliation:
São Paulo State University (UNESP), Institute of Biosciences, Section of Parasitology, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
G. Pérez-Ponce de León
Affiliation:
Escuela Nacional de Estudios Superiores Unidad Mérida (ENES)-UNAM, Km 4.5 Carretera Mérida-Tetiz, Ucú, Yucatán, Mexico
R. Silva
Affiliation:
São Paulo State University (UNESP), Institute of Biosciences, Section of Parasitology, Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo CEP 18618-689, Brazil
*
Corresponding author: Enzo Emmerich; Email: [email protected]
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Abstract

Ochetosoma heterocoelium (Travassos, 1921) was collected from the mouth and oesophagus of a pit viper, Bothrops moojeni Hoge, 1966 in São Sebastião do Paraíso Farm (21°51’48.26" S, 48°26’56.78" W), municipality of Boa Esperança do Sul, São Paulo State, Brazil. In this study, we provide the first molecular characterisation of this digenean using 28S rDNA and COI sequences, and its phylogenetic position within the Plagiorchiida is assessed. Furthermore, new morphological features are added to the diagnosis of the species, and scanning electron microscopy photomicrographs are presented. Sequences of the 28S rRNA gene of O. heterocoelium were successfully obtained and aligned with 35 digenean species belonging to Plagiorchiida. Only three congeners – O. aniarum (Leidy, 1890), O elongatum (Seo et al. 2024), and O. kansense (Crow, 1913) – have been sequenced for this molecular marker. The newly sequenced individuals of O. heterocoelium are 98.7% and 99.4% similar to O. aniarum and O. kansensis, respectively, and Ochetosoma is not recovered as a monophyletic group. Dasymetra nicolli Holl and Allison, 1935 and Lechriorchis tygarti Talbot, 1933 are nested with Ochetosoma.

Keywords

TrematodaDicrocoeliidaePlagiorchiidae28S rDNAphylogeny
Type
Research Paper
Information
Journal of Helminthology , Volume 99 , 2025 , e57
Copyright
© The Author(s), 2025. Published by Cambridge University Press

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