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Cytotoxicity and Biokinetic Evaluation of Clay Minerals

Published online by Cambridge University Press:  01 January 2024

Soo-Jin Choi
Soo-Jin Choi*
Affiliation:
Division of Applied Food System, Major of Food Science & Technology, Seoul Women’s University, Hwarang-ro 621, Nowon-gu, Seoul 01797, Republic of Korea
*
*[email protected]
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Abstract

Clay minerals, such as layered double hydroxide (LDH) and montmorillonite (MMT), have attracted a great deal of attention for biological applications. Along with the rapid development of nanotechnology, public concern about the potential toxicity of nanoparticles is growing. In the present work, cytotoxicity of LDH and MMT was assessed in terms of inhibition of cell proliferation, generation of oxidative stress, and induction of inflammation response. Moreover, the biokinetics of LDH and MMT were evaluated; biokinetics provides information about in vivo absorption, distribution, and excretion kinetics. The results demonstrated that both LDH and MMT inhibited cell proliferation at relatively large concentrations and after long exposure time compared to other inorganic nanoparticles, although they generated reactive oxygen species (ROS). LDH induced pro-inflammatory cytokines in a size-dependent manner. Biokinetic study revealed that, after single-dose oral administration to mice, both LDH and MMT had extremely slow oral rates of absorption and did not accumulate in any specific organ. All the results suggest great potential of clay minerals for biological application at safe levels.

Keywords

AbsorptionDistributionExcretionInflammationLayered double hydroxideMontmorilloniteOxidative stress
Type
Research Article
Information
Clays and Clay Minerals , Volume 67 , Issue 1 , 15 February 2019 , pp. 91 - 98
Copyright
Copyright © Clay Minerals Society 2019

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