Glufosinate resistance was previously confirmed in three Palmer amaranth accessions from Arkansas (MSR1, MSR2, and CCR). Greenhouse screening results suggested the presence of multiple herbicide resistance. Therefore, this study aimed to determine the postemergence resistance profile of these three glufosinate-resistant Palmer amaranth accessions. Field experiments were also conducted to assess preemergence and postemergence herbicide options to control the accession with the highest glufosinate resistance level (MSR2). A dose-response assay with the three resistant accessions and two susceptible standards was conducted with the herbicides 2,4-D, atrazine, dicamba, diuron, fomesafen, glyphosate, imazethapyr, and mesotrione. The preemergence and postemergence field experiments with MSR2 evaluated 15 and 16 single active ingredients, respectively. The Palmer amaranth accessions that carried glufosinate resistance were also confirmed to be resistant to six other postemergence herbicides: 2,4-D, diuron, fomesafen, glyphosate, imazethapyr, and mesotrione. CCR is also resistant to dicamba. Therefore, accessions MSR1, MSR2, and CCR have evolved resistance to postemergence herbicides pertaining to seven sites of action. A shift toward increased tolerance to atrazine has also been observed among all resistant accessions. Overall, field preemergence treatments with atrazine, pyroxasulfone, or trifludimoxazin obtained the highest MSR2 control levels at all evaluation times and the lowest number of seedlings emerging at 3 and 6 wk after treatment. In the postemergence experiment, only paraquat obtained MSR2 control levels above 90% at all ratings. The lowest number of alive MSR2 plants was observed after postemergence treatments with paraquat or trifludimoxazin. Fields near where glufosinate resistance has been confirmed in Palmer amaranth will likely demand a more diverse and proactive management strategy that relies on combinations of chemical, cultural, and mechanical control tactics. Future efforts should focus on sequential applications and mixture, the elucidation of all resistance mechanisms in the evaluated accessions, and soil-applied dose-response.
